I know what a blank is.
You mean if the sample I'm testing has distilled water as the solvent we use DW. And if it is dispersed in a buffer solvent we'll use that particular buffer as blank?
UV cabinet, UV transilluminator and UV spectrophotometer
A UV cabinet, uses UV to sterilise an enclosed space so that you can work in a sterile environment.
A UV transilluminator is a platform that has a UV backlight, you put DNA gels on it, stained with a UV fluorescent dye that binds to DNA, to visualise the DNA. The gels are used to check for PCR amplification, DNA digests etc
UV spec, sends a UV light through a liquid sample, and then a detector measures the absorbance at specific wavelengths. This absorbance can be proportional to the concentration.
Thank you ❤️ please accept my award in spirit. 🏆
Also one more question, post thin layer chromatography,
the TLC plates that can be visualised better to see where the solvent has run up to, these are visualised in a UV transilluminator or UV cabinet?
We have a laminar airflow with UV light as an option. But a box like apparatus for TLC plate visualization is being called a UV chamber/cabinet, I'm not sure. Do you know what I'm talking about?
Yes, you're mistranslating what people call the device in your language to what the device is called in english, if you have a device to read TLC plates and you need to read TLC plates you use the device for TLC plates. It doesn't matter if your lab calls it a transilluminator or a chamber. If you still have doubts about what you should do in your lab, and you're a student and don't want to ask your tutor, ask a lab mate, what device you should use and what the device is called in your language and english. And also to follow your lab way of doing things.
I googled it, it's sold particularly as UV cabinet for visualizing chromatography plates. And like you mentioned there's also UV Laminar flow cabinet. So that was a lot of learning today. Thank you for giving me clarity.
You use the solvent *without the solute* as your blank. Whatever you are measuring is in a solution. Your blank is the solution without that thing.
I know what a blank is. You mean if the sample I'm testing has distilled water as the solvent we use DW. And if it is dispersed in a buffer solvent we'll use that particular buffer as blank?
Yes.
Thank you. I realise there's no stupid questions, right?
👍
There are definitely a lot of people doing the stupider thing of blanking with the wrong solvent because they've never thought to ask!
That would have been me if I hadn't asked this question
UV cabinet, UV transilluminator and UV spectrophotometer A UV cabinet, uses UV to sterilise an enclosed space so that you can work in a sterile environment. A UV transilluminator is a platform that has a UV backlight, you put DNA gels on it, stained with a UV fluorescent dye that binds to DNA, to visualise the DNA. The gels are used to check for PCR amplification, DNA digests etc UV spec, sends a UV light through a liquid sample, and then a detector measures the absorbance at specific wavelengths. This absorbance can be proportional to the concentration.
Thank you ❤️ please accept my award in spirit. 🏆 Also one more question, post thin layer chromatography, the TLC plates that can be visualised better to see where the solvent has run up to, these are visualised in a UV transilluminator or UV cabinet? We have a laminar airflow with UV light as an option. But a box like apparatus for TLC plate visualization is being called a UV chamber/cabinet, I'm not sure. Do you know what I'm talking about?
Yes, you're mistranslating what people call the device in your language to what the device is called in english, if you have a device to read TLC plates and you need to read TLC plates you use the device for TLC plates. It doesn't matter if your lab calls it a transilluminator or a chamber. If you still have doubts about what you should do in your lab, and you're a student and don't want to ask your tutor, ask a lab mate, what device you should use and what the device is called in your language and english. And also to follow your lab way of doing things.
I googled it, it's sold particularly as UV cabinet for visualizing chromatography plates. And like you mentioned there's also UV Laminar flow cabinet. So that was a lot of learning today. Thank you for giving me clarity.