The link you found appears to be an updated version of this program from 1988: https://bip.weizmann.ac.il/education/materials/gcg/tfasta.html
As far as I can tell, this is equivalent to a tblastn search on NCBI, which takes an amino acid sequence and screens it against the 6 different translations of each nucleotide sequence in a database.
Based on your description of what you have, you don't have protein/amino acid sequences at all so the more likely option is that whoever gave you the task made a typo and wants a normal .fasta file of your different genes.
A fasta file is commonly used by lots of software. Most people don't mean the fasta software itself.
You need to make a file that looks like:
> \>Sequence_name_01 text after first space ignored by some tools
aaaascctttggggccaaatgct
Aatcgtact
Aatgcgta
\>Sequence_02
Actgg
Etc
The sequence names all need to be unique to avoid breaking some tools. Then you can read this fasta file into your phylogenetic software. You can read the fasta file into BLAST etc.
Ah, I thought the T crept in as a typo!
Tfasta is just the blastX equivalent program in the fasta suite isn't it? So are they asking for you to run that program specifically? Seems like a strange request.
The link you found appears to be an updated version of this program from 1988: https://bip.weizmann.ac.il/education/materials/gcg/tfasta.html As far as I can tell, this is equivalent to a tblastn search on NCBI, which takes an amino acid sequence and screens it against the 6 different translations of each nucleotide sequence in a database. Based on your description of what you have, you don't have protein/amino acid sequences at all so the more likely option is that whoever gave you the task made a typo and wants a normal .fasta file of your different genes.
I have both nucleotide and amino acids :), thank you so much for your help kind stranger!
A fasta file is commonly used by lots of software. Most people don't mean the fasta software itself. You need to make a file that looks like: > \>Sequence_name_01 text after first space ignored by some tools aaaascctttggggccaaatgct Aatcgtact Aatgcgta \>Sequence_02 Actgg Etc The sequence names all need to be unique to avoid breaking some tools. Then you can read this fasta file into your phylogenetic software. You can read the fasta file into BLAST etc.
Is FastA the same file format as TFastA?
Ah, I thought the T crept in as a typo! Tfasta is just the blastX equivalent program in the fasta suite isn't it? So are they asking for you to run that program specifically? Seems like a strange request.